Cancer Terms

Yeast One Two-Hybrid System

Cancer Terms -> Activity -> Techniques -> Research Techniques -> Molecular Biology Techniques -> Expression Technologies for DNA and RNA -> Yeast One Two-Hybrid System

Yeast One Two-Hybrid System Definition

Molecular biology technique used to identify interacting proteins in a yeast model. A protein of interest (the bait) is fused to the DNA-binding domain of a yeast transcription factor (usually GAL4). (Usually) a cDNA library is fused to the GAL4 activation domain. The library is then introduced into yeast cells carrying the bait construct. Functional interaction of the bait with fusion proteins from the library bring together the binding and activation domains of the transcription factor, allowing activation of a reporter gene. (syn: yeast two-hybrid system) (NCI/OSP)

Yeast One Two-Hybrid System Synonyms

Yeast One/Two-Hybrid System, Two Hybrid, Yeast One Hybrid System, Yeast Two Hybrid System

Terms in Yeast One Two-Hybrid System category



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