Cancer Terms

cDNA Library Construction

Cancer Terms -> Activity -> Techniques -> Research Techniques -> Molecular Biology Techniques -> Cloning -> Gene Cloning -> cDNA Library Construction

cDNA Library Construction Definition

Methods for building collections of cloned DNA consisting of DNA copies of all the mRNAs produced by a cell type. cDNAs are generated using reverse transcriptase and either an oligo-dT or random primer. The copies are inserted into a bacterial plasmid or bacteriophage vector. The library can be maintained as a pooled population or as an arrayed library.

cDNA Library Construction Synonyms

cDNA Library Construction

Terms in cDNA Library Construction category



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